Journal of Clinical Medicine Research, ISSN 1918-3003 print, 1918-3011 online, Open Access
Article copyright, the authors; Journal compilation copyright, J Clin Med Res and Elmer Press Inc
Journal website https://www.jocmr.org

Original Article

Volume 14, Number 7, July 2022, pages 273-281

High Expression of Heat Shock Protein Family D Member 1 Predicts Poor Prognosis of Esophageal Cancer

Figures

Figure 1.
Figure 1. Expression of HSPD1 in esophagus cancer patients based on TCGA data. The expression level of HSPD1 in esophagus cancer tissues was significantly higher than that in adjacent non-neoplastic tissues (P = 0.0237). HSPD1: heat shock protein family D (Hsp60) member 1; TCGA: The Cancer Genome Atlas.
Figure 2.
Figure 2. Immunohistochemical analysis of the HSPD1 expression in esophagus cancer and adjacent non-neoplastic tissues. Positive expression of HSPD1 was mainly found in the cytoplasm (magnification, × 400). HSPD1: heat shock protein family D (Hsp60) member 1.
Figure 3.
Figure 3. Association of HSPD1 expression with clinical variables based on TCGA data. (a) Age. (b) Gender. (c) Histological type. (d) Clinical stage. (e) T classification. (f) N classification. (g) M classification. HSPD1: heat shock protein family D (Hsp60) member 1; TCGA: The Cancer Genome Atlas.
Figure 4.
Figure 4. The prognostic significance of HSPD1 in esophagus cancer. Kaplan-Meier method and log-rank test were performed based on expression level of HSPD1 in esophagus cancer cohort from TCGA database (a) and our study (b). HSPD1: heat shock protein family D (Hsp60) member 1; TCGA: The Cancer Genome Atlas.
Figure 5.
Figure 5. Forest plot of multivariate Cox regression analyses of overall survival in esophagus cancer cohort from TCGA database (a) and our study (b). TCGA: The Cancer Genome Atlas.
Figure 6.
Figure 6. KEGG enrichment plots from GSEA. The GSEA results revealed that genes involved in cysteine and methionine metabolism, spliceosome, selenoamino acid metabolism, mismatch repair, RNA degration, DNA replication and cell cycle were differentially enriched in HSPD1-associated esophagus cancer. HSPD1: heat shock protein family D (Hsp60) member 1; GSEA: gene set enrichment analysis.

Tables

Table 1. HSPD1 Expression in Esophagus Cancer Tissues and Adjacent Non-Neoplastic Tissues
 
GroupnHSPD1 expressionP value
Low (%)High (%)
*P < 0.05. HSPD1: heat shock protein family D (Hsp60) member 1.
Normal2018 (90%)2 (10%)< 0.001*
Tumor8740 (46%)47 (54%)

 

Table 2. Logistic Regression of HSPD1 Expression and Clinicopathological Characteristics in TCGA Database
 
Clinical characteristicsnOdds ratio in expressionP value
*P < 0.05. HSPD1: heat shock protein family D (Hsp60) member 1; TCGA: The Cancer Genome Atlas.
Age
  < 65 vs. ≥ 651590.995 (0.520 - 1.92)0.893
Gender
  Female vs. male1591.359 (0.56 - 3.39)0.45
Pathology type
  Adenocarcinoma vs. squamous carcinoma1580.488 (0.257 - 0.916)0.027*
Stage
  I-II vs. III-IV1401.823 (0.923 - 3.646)0.086
T
  T0-2 vs. T3-41441.863 (0.962 - 3.648)0.067
N
  N0 vs. N1-31422.109 (1.083 - 4.167)0.029*
M
  M0 vs. M11287.875 (1.343 - 149.581)0.057

 

Table 3. Associations Between HSPD1 Expression and Clinicopathological Characteristics in Our Esophagus Cancer Patients
 
CharacteristicsnHSPD1 protein expressionP value
Low (%)High (%)
*P < 0.05. HSPD1: heat shock protein family D (Hsp60) member 1.
Age0.996
  ≥ 655023 (46.0)27 (54.0)
  < 653717 (45.9)20 (54.1)
Stage0.001*
  I-II4227 (64.3)15 (35.7)
  III-IV4513 (28.9)32 (71.1)
T0.381
  T0-T25025 (50.0)25 (50.0)
  T3-T43715 (40.5)22 (59.5)
N0.014*
  N04225 (59.5)17 (40.5)
  N1-N34515 (33.3)30 (66.7)
M0.335
  M08038 (47.5)42 (52.5)
  M172 (28.6)5 (71.4)
Histological type0.116
  Squamous carcinoma7537 (49.3)38 (50.7)
  Adenocarcinoma123 (25.0)9 (75.0)
Histological grade< 0.001*
  Well4027 (67.5)13 (32.5)
  Moderately/poorly4713 (27.7)34 (72.3)