J Clin Med Res

Journal of Clinical Medicine Research, ISSN 1918-3003 print, 1918-3011 online, Open Access

Article copyright, the authors; Journal compilation copyright, J Clin Med Res and Elmer Press Inc

Journal website http://www.jocmr.org

Original Article

Volume 9, Number 5, May 2017, pages 416-425

Role of LncRNA-AF085935, IL-10 and IL-17 in Rheumatoid Arthritis Patients With Chronic Hepatitis C

**Table 1.** Demographic Characters in Different Study Groups
Variables	Cases (n = 65), mean ± SD	Control (n = 30), mean ± SD	P-value
Age (years)	39.4 ± 11.7	41.5 ± 8.4	0.4
BMI	27.6 ± 2.1	26.1 ± 3	0.1
Sex, no. (%)
Male	47 (72.3%)	19 (63.3%)	0.5
Female	18 (27.7%)	11 (36.7%)

Table 1. Demographic Characters in Different Study Groups

Variables

Cases (n = 65), mean ± SD

Control (n = 30), mean ± SD

P-value

Age (years)

39.4 ± 11.7

41.5 ± 8.4

0.4

BMI

27.6 ± 2.1

26.1 ± 3

0.1

Sex, no. (%)

Male

47 (72.3%)

19 (63.3%)

0.5

Female

18 (27.7%)

11 (36.7%)

**Table 2.** Laboratory Investigations in Different Studied Groups
Variables	Cases (n = 65), mean ± SD	Control (n = 30), mean ± SD	P-value
AST	45.1 ± 14.6	27.1 ± 7.5	< 0.001
ALT	52.7 ± 10.6	26.3 ± 7.8	< 0.001
Total bilirubin	0.74 ± 0.16	0.96 ± 0.21	< 0.001
Direct bilirubin	0.23 ± 0.11	0.26 ± 0.14	0.2
Albumin	4.1 ± 0.48	4.3 ± 0.59	0.06
Alkaline phosphate	68.9 ± 24.6	81.9 ± 28.2	0.02
PC	0.88 ± 0.07	0.91 ± 0.06	0.02
INR	1.08 ± 0.10	1.1 ± 0.12	0.3
Creatinine	0.93 ± 0.29	0.93 ± 0.29	0.9
HbA1c	5.4 ± 0.78	5.33 ± 0.94	0.8
TSH	1.63 ± 0.98	1.89 ± 1.5	0.3
AFP	13.2 ± 6.3	3.33 ± 2.1	< 0.001
RBS	87.6 ± 23.3	95.7 ± 26.2	0.1
Hb (g)	13.2 ± 2.3	10.8 ± 1.1	< 0.001
TLC (× 1,000)	6.4 ± 1.9	4.2 ± 1.2	< 0.001
Platelets	273.6 ± 103.5	181.1 ± 69.4	< 0.001

Table 2. Laboratory Investigations in Different Studied Groups

Variables

Cases (n = 65), mean ± SD

Control (n = 30), mean ± SD

P-value

AST

45.1 ± 14.6

27.1 ± 7.5

< 0.001

ALT

52.7 ± 10.6

26.3 ± 7.8

< 0.001

Total bilirubin

0.74 ± 0.16

0.96 ± 0.21

< 0.001

Direct bilirubin

0.23 ± 0.11

0.26 ± 0.14

0.2

Albumin

4.1 ± 0.48

4.3 ± 0.59

0.06

Alkaline phosphate

68.9 ± 24.6

81.9 ± 28.2

0.02

0.88 ± 0.07

0.91 ± 0.06

0.02

INR

1.08 ± 0.10

1.1 ± 0.12

0.3

Creatinine

0.93 ± 0.29

0.9

HbA1c

5.4 ± 0.78

5.33 ± 0.94

0.8

TSH

1.63 ± 0.98

1.89 ± 1.5

0.3

AFP

13.2 ± 6.3

3.33 ± 2.1

< 0.001

RBS

87.6 ± 23.3

95.7 ± 26.2

0.1

Hb (g)

13.2 ± 2.3

10.8 ± 1.1

< 0.001

TLC (× 1,000)

6.4 ± 1.9

4.2 ± 1.2

< 0.001

Platelets

273.6 ± 103.5

181.1 ± 69.4

< 0.001

**Table 3.** HCV RNA, IL-10, IL-17 and lncRNA-AF085935 in Different Study Groups
Variables	G1: before treatment (n = 65), mean ± SD	G2: after treatment (n = 65), mean ± SD	G3: control (n = 30), mean ± SD	P-value
^aSignificance between G1 and G2. ^bSignificance between G1 and G3. ^cSignificance between G2 and G3.
HCV RNA	323,955 ± 337,313.6	1418,729.7 ± 2,380,203.2		0.001^a
IL-10	68.5 ± 24.7	201.4 ± 31.9	51.9 ± 20.3	< 0.001^{a, b, c}
IL-17	227.9 ± 35.5	112.4 ± 28.1	53.4 ± 20.7	< 0.001^{a, b, c}
LncRNA-AF085935	4.12 ± 0.67	4.17 ± 0.66	1.84 ± 1.11	< 0.001^{b, c}

Table 3. HCV RNA, IL-10, IL-17 and lncRNA-AF085935 in Different Study Groups

Variables

G1: before treatment (n = 65), mean ± SD

G2: after treatment (n = 65), mean ± SD

G3: control (n = 30), mean ± SD

P-value

^aSignificance between G1 and G2. ^bSignificance between G1 and G3. ^cSignificance between G2 and G3.

HCV RNA

323,955 ± 337,313.6

1418,729.7 ± 2,380,203.2

0.001^a

IL-10

68.5 ± 24.7

201.4 ± 31.9

51.9 ± 20.3

< 0.001^{a, b, c}

IL-17

227.9 ± 35.5

112.4 ± 28.1

53.4 ± 20.7

< 0.001^{a, b, c}

LncRNA-AF085935

4.12 ± 0.67

4.17 ± 0.66

1.84 ± 1.11

< 0.001^{b, c}

**Table 4.** Correlation Between HCV RNA Level With Other Parameters Before and After Treatment Among Study Group
Variables	r (P-value)
IL-10	0.02 (0.8)	0.01 (0.9)
IL-17	0.05 (0.7)	-0.17 (0.2)
LncRNA-AF085935	-0.033 (0.9)	0.04 (0.8)

Table 4. Correlation Between HCV RNA Level With Other Parameters Before and After Treatment Among Study Group

Variables

r (P-value)

HCV-PCR before treatment

HCV-PCR after treatment

IL-10

0.02 (0.8)

0.01 (0.9)

IL-17

0.05 (0.7)

-0.17 (0.2)

LncRNA-AF085935

-0.033 (0.9)

0.04 (0.8)

**Table 5.** Sensitivity and Specificity of Different Parameters in Predicting the Efficacy of Steroid Therapy in Patients
Variable	Sensitivity	Specificity	Accuracy	Cut-off point
IL-10
Before	77.3%	23.3%	51%	45
After	72.7%	30.2%	53.9%	175.1
IL-17
Before	72.7%	25.6%	42%	198.5
After	81.8%	23.3%	62.1%	196.8
LncRNA-AF085935
Before	63.6%	27.2%	48.5%	3.74
After	68.2%	20.9%	38.4%	3.63

Table 5. Sensitivity and Specificity of Different Parameters in Predicting the Efficacy of Steroid Therapy in Patients

Variable

Sensitivity

Specificity

Accuracy

Cut-off point

IL-10

Before

77.3%

23.3%

51%

After

72.7%

30.2%

53.9%

175.1

IL-17

Before

72.7%

25.6%

42%

198.5

After

81.8%

23.3%

62.1%

196.8

LncRNA-AF085935

Before

63.6%

27.2%

48.5%

3.74

After

68.2%

20.9%

38.4%

3.63